The present work describes a simple reverse phase HPLC method for the determination of omeprazole and domperidone from capsule formulations. The determination was carried out on a C-18 (250× 4.6 mm, 5 micron) column using mobile phase contains 35 volumes of acetonitrile and 65 volume a solution of 0.1M ammonium acetate (anhydrous). The flow rate and runtime were 1 ml/min and 20 min, respectively. The eluent was monitored at 286 nm. The method was reproducible, with good resolution between omeprazole and domperidone. The detector response was found to be linear in the concentration range of 10-30 μg/ml for omeprazole and 5-15 μg/ml for domperidone.
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